61135-33-9

Description

5-Ethynyl-2′-deoxyuridine (61135-33-9) is a novel agent for fast and sensitive detection of DNA synthesis?in vivo.1 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation.1 In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-Ethynyl-2′-deoxyuridine may be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis.2

Uses

5-Ethynyl-2’deoxyuridine is a extensively used nucleoside analog used as a probe for DNA synthesis. However, 5-Ethynyl-2’deoxyuridine is a toxic anti-metabolite, which potentially causes DNA instabi liy, necrosis and cell-cycle arrest, therefore it is often used for short-term DNA synthesis in tissue culture and living organisms where prolonged cell survival is not required.